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. 2013 Mar 1;3(2):e24542. doi: 10.4161/mge.24542

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Copyright © 2013 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name mge-3-e24542-g1.jpg — Figure 1. The distribution of full-length Penelope copies and Penelope-derived transcripts in various strains and species of Drosophila. (A) The presence of intact and potentially active Penelope copies and canonical (2.8 kb) Penelope transcripts in different Drosophila strains and species. The position of intron is indicated. Black arrow indicates the transcription start. *In our experiments we obtained strains transformed with Penelope using both D. virilis and D. melanogaster. TR-terminal repeats which can be in tandem or inverse orientation in different Penelope copies. RT-reverse transcriptase; EN-endonuclease. Cleavage sites of XhoI endonuclease are indicated. (B) Penelope-homologous small RNAs detected in the virilis group species, certain D. virilis strains and a few strains transformed with Penelope. Maternally transmitted Penelope-derived piRNAs may target Penelope mRNA through transcript cleavage.