Figure 1. The N-terminus of E1A specifically requires hBre1 for full activation of transcription.

U-2 OS cells were transfected with a negative control siRNA or 1 of 4 siRNAs specific for hBre1. Cells were then transfected with a constitutive β-galactosidase reporter, a Gal4 responsive luciferase reporter and a vector expressing the Gal4 DNA binding domain (DBD) alone, the Gal4 DBD fused to the N-terminus of E1A, or the Gal4 DBD fused to E1A CR3. Luciferase activity was measured. Results were normalized to β-galactosidase activity and siRNA treated groups were set as a fold change to the Gal4 only transfected counterpart. A statistically significant decrease from control siRNA treatment is indicated (* P<0.01). n = 3.