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. 2013 Jun 13;9(6):e1003411. doi: 10.1371/journal.ppat.1003411

Figure 4. The N-terminus of E1A, but not CR3 requires hPaf1 for efficient transcriptional activation.

Figure 4

U-2 OS cells were transfected with a negative Ctrl siRNA or siRNAs specific for known hBre1 interacting proteins. Cells were then transfected with a constitutive β-galactosidase reporter, a Gal4 responsive luciferase reporter and a vector expressing the Gal4 DBD alone, Gal4 DBD fused to the N-terminus of E1A, or Gal4 DBD fused to E1A CR3. Luciferase activity was measured. Results were normalized to β-galactosidase activity and siRNA treated groups were set as a fold to the Gal4 only transfected counterpart. A statistically significant decrease from Ctrl siRNA treatment is indicated (* P<0.01). n = 3.