Figure 9. Drugs affecting cytoskeleton and membrane cholesterol prevent stimulation of pDC by infected cells.

A. SK-6 cells were infected with VRPΔE^rns for 24 h, washed and then treated with a DMSO control, nocodazole (5 µM), MβCD (20 mM) or latrunculin (1 µM) for 2 h at 37°C. The inhibitors were then removed and the cells washed three times to avoid effects on pDC. The treated cells were co-cultured with pDC and IFN-α measured after 20 h of incubation. B. Uninfected SK-6 cells were treated as in A and co-cultured with pDC in presence of CpG. The mean values of three replicates with standard deviation are shown. The data is representative of three independent experiments.