Figure 5. Comparison of qPCR and microarray results following Herbimycin A-treatment.

Transcriptional changes from the Herb A-treatment of adult schistosome females detected in qPCR and microarray data were calculated as log₂ratios (treated/control). According to the microarray analysis SmActRIIb, calmodulin-4, and hsp70 showed enhanced transcription (A), whereas tetraspanin-1, SmSmad 4, and Smp48 were detected as transcriptionally repressed (B). For each method three biological replicas were used, each with two technical replicas for the microarray analysis (except microarray 2) and three technical replicas for the qPCR. The mean of the technical replicas was calculated and is presented with standard deviations.