Figure 6. Comparison of qPCR and microarray results following the combined inhibitor treatment.

Transcriptional changes from the simultaneous TRIKI- and Herb A-treatment of adult schistosome females detected in qPCR and microarray data were calculated as log₂ratios (treated/control). The investigated genes with enhanced transcription, as determined by microarray analysis, were calmodulin-4 and hsp70 (A), the genes with repressed transcription comprised a gene similar to fs800, an eggshell precursor protein, tetraspanin 1, SmTYR1, and cathepsin S (B). For each method three biological replicas were used, each with two technical replicas for the microarray analysis (except microarray 2) and three technical replicas for the qPCR. The mean of the technical replicas was calculated and is presented with standard deviations.