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. 2013 Jun 13;8(6):e66170. doi: 10.1371/journal.pone.0066170

Figure 5. Analysis of neurite length on different pillar parameters.

Figure 5

(A) Axon and average neurite length at different time points after plating. The black line shows the growth evolution on a flat substrate, red shows W = 4 µm, S = 2 µm, blue shows W = 1.8 µm, S = 1.6 µm. The process lengths for W = 1.8 µm, S = 1.6 µm, and W = 4 µm, S = 2 µm are significantly (p<0.05) higher than on flat after 4, 8, 12 and 20 h. (B) Examples of neuronal morphology on flat and on the previously summed pillar parameters. Scale bars are 10 µm. (C) Surface plot of axon length in function of width and spacing after 20 h. Maximal axon length at 20 h is achieved by pillars of W = 1–2 µm and S = 1–2 µm. (D) Time lapse imaging (GFP-UtrCH) of a minor neurite extending its processes. See Movie S1 for full time lapse. W = 2.8 µm, S = 1.6 µm. Scale bar is 10 µm.