Table 3. Ranking of reference gene candidates, based on stability of expression in Arctic charr whole embryos using either qPCR or RNA-seq.
| qPCR | RNA-seq | ||||
| Gene | Nf | SD | C.V. | p M | p T |
| ACTB | 2 | 3 | 4 | * | - |
| b2m | 11 | 11 | 11 | ** | ** |
| EF1α | 3 | 4 | 6 | - | - |
| HPRT | 5 | 5 | 3 | - | * |
| IF5A1 | 10 | 10 | 9 | - | ** |
| RL7 | 9 | 9 | 7 | - | - |
| RS9 | 1 | 2 | 8 | - | * |
| RS20 | 7 | 8 | 10 | * | ** |
| TBA | 8 | 6 | 5 | - | - |
| UB2L3 | 4 | 1 | 1 | - | - |
| UBIQ | 6 | 7 | 2 | - | - |
Abbreviations: Nf _ NormFinder, SD = standard deviation, C.V. = coefficient of variation (used to rank), p M = significant differences between morphological groups, p T = significant differences between developmental time points.
**p = <0.01; * = p<0.05; - = no significant difference.