Figure 2. Averaged traces of laser Doppler flowmetry in C57BL/6J and IP₃R2-KO mice.

Results for C57BL/6J and IP₃R2-KO mice are represented in blue and red traces, respectively. Each experimental condition has N>5 animals. Upon 200 µA stNBM, the cerebral blood flow (CBF) showed an immediate increase, followed by a transient decrease that overshot the baseline both in WT (A) and KO (E). CBF change by the stNBM is attenuated by the muscarinic receptor antagonist atropine (ATR) (B). Weak stNBM stimulation (50 µA) resulted in negative laser Doppler flowmetry signal (C) and similar changes were observed in KO (F). Stimulation outside the NBM failed to induce CBF increase (D). Shaded areas represent s.e.m.