Figure 7. IDO attenuates T_H17 responses to PR8 challenge.

X31 primed WT and IDO1-KO (B6) mice were challenged with PR8 and CD4 T cell status were assessed by FACS analysis of gated CD4 T cells in BAL and lung parenchyma at 5 dpi after ex vivo stimulation with PMA/ionomycin and staining to detect intracellular cytokines. A. Representative FACS plots for intracellular staining of IL17a and IFNγ in gated CD4 T cells. BC. Percentages of IL17a (B) and IFNγ⁺ (C) CD4 T cells are shown. Data were generated from mice primed from various times but re-challenged in the same experiment (2–3/group). Data were analyzed using Student's unpaired t test; **p<0.01, NS: non significant.