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. 2013 Jun 13;8(6):e65734. doi: 10.1371/journal.pone.0065734

Figure 1. Effect of triol on viability and proliferation of human prostate cancer cell lines.

Figure 1

LNCaP CDXR-3, DU-145, and PC-3 prostate cancer cells were treated with increasing concentrations of triol for 48 hrs (A)(C) or 96 hrs (B)(D). Relative viability and relative cell number of prostate cancer cells was determined by MTT (3,4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) 96-well assay (A)(B) and Hoechst dye 33258-based 96-well proliferation assay (C)(D), respectively, as described in Materials and Methods. Cell numbers were normalized to control (dimethylsulfoxide) of each cell line. Asterisk (*, **, ***) represents statistically significant difference (p<0.05, p<0.01, p<0.001) between the treated group and the control group.