Figure 3. Effect of triol on the growth of PC-3 xenografts in nude mice.
Male Balb/c nu/nu mice, 6–8 weeks of age, were injected subcutaneously in both flanks with 5×105 PC-3 cells suspended in 0.5 ml of Matrigel. Tumors were measured daily using calipers and volume was calculated using the formula volume = length×width×height×0.52. Monitoring of tumor growth was started one week after tumor inoculation. Mice were separated into control and treatment groups. The control group had 5 mice carrying 10 tumors and the treatment group had 5 mice carrying 8 tumors. Three weeks after the initial inoculation, the treatment group was orally administrated triol daily at a dose of 20 mg/kg, 5 days/week. Mice in the control group were gavaged with vehicle only. Treatment started on day 22 and ended on day 42 after tumor inoculation. Tumor volumes were shown as mean ± standard error (A) while body weight of mice was shown as mean ± Standard Deviation (B).