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. 2013 Jun 13;8(6):e65734. doi: 10.1371/journal.pone.0065734

Figure 11. Effect of triol on LXR reporter and ABCA1 gene expression.

Figure 11

(A) PC-3 cells were transfected with pRL-TK-Renilla luciferase plasmid, pSG5RXRα, pSG5LXRα, and 4xDR4Δ56cfos pGL3 reporter gene plasmid. PC-3 cells were treated with 0, 5, 10, 20 µM triol or 1 µM T0901317. Luciferase reporter gene assay was performed to determine the activity of LXRα receptor. (B) PC-3 and DU-145 cells were treated with 0, 10, 20 µM triol for 48 hrs. ABCA1 gene, a target gene of LXRα, was determined by qRT-PCR. Asterisk * represents statistically significant difference (p<0.05) between the control and triol treatment group.