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. 2013 Jun 13;8(6):e65734. doi: 10.1371/journal.pone.0065734

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© 2013 Lin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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PC-3, DU-145, and CDXR-3 cells were pre-treated with 0, 10, or 20 µM triol for 48 hrs. Cells were then counted and seeded at identical number. Cancer cell migration ability (A) and invasion ability (B) was determined by transwell migration assay (A) and transwell invasion assay 6 hrs (A) and 24 hrs (B) after cell seeding, respectively. (C) Migration of CDXR-3 cells was determined by transwell migration assay 24 hrs after cell seeding. There was no triol in medium during the migration or invasion assay. Asterisk *** represents statistically significant difference (p<0.001) between the control group and triol treatment group.