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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Mol Cancer Ther. 2013 Mar 29;12(6):1099–1111. doi: 10.1158/1535-7163.MCT-12-0737

Figure 5.

Figure 5

Examination of MT network and polymerization in SKOV3.ip1 stable cells in the presence or absence of paclitaxel. A, MT network was examined by immunofluorescence staining (left panel) and fluorescence intensity quantification (right panel) in SKOV3.ip1 stable cells using anti-α-tubulin antibody after a 12-hour incubation in the absence or presence of paclitaxel. Scale bar, 100 μm. B, Soluble (S) and polymerized (P) α- and β-tubulin subunits were isolated from SKOV3.ip1 stable cells after a 12-hour incubation with paclitaxel and then analyzed by western blotting (left panel) and densitometric quantification (middle and right panels). β-actin served as a loading control. Vector, SKOV3.ip1-vector; AA, SKOV3.ip1-AA (stably expressing nonphosphorylatable PEA-15 at Ser104 and Ser116, which were substituted with alanine); DD, SKOV3.ip1-DD (stably expressing phosphomimetic PEA-15 at Ser104 and Ser116, which were substituted with aspartic acid). *, P < 0.05; **, P < 0.01; #, P < 0.001.