Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Mol Cancer Ther. 2013 Mar 29;12(6):1099–1111. doi: 10.1158/1535-7163.MCT-12-0737

Figure 6.

Figure 6

Examination of detyrosinated (Glu) and acetylated (Ac) α-tubulin expression in SKOV3.ip1 stable cells in the presence or absence of paclitaxel. Expression of (A) Glu and (B) Ac α-tubulin was examined by immunofluorescence staining (upper panels) and fluorescence intensity qualification (lower panels) in SKOV3.ip1 stable cells after a 1-hour incubation in the absence or presence of paclitaxel. Scale bar, 10 μm. Expression of (C) Glu and (D) Ac α-tubulin was examined by western blotting (upper panels) and densitometric quantification (lower panels) in SKOV3.ip1 stable cells after a 48-hour incubation with paclitaxel. Vector, SKOV3.ip1-vector; AA, SKOV3.ip1-AA (stably expressing nonphosphorylatable PEA-15 at Ser104 and Ser116, which were substituted with alanine); DD, SKOV3.ip1-DD (stably expressing phosphomimetic PEA-15 at Ser104 and Ser116, which were substituted with aspartic acid). *, P < 0.05; **, P < 0.01; #, P < 0.001.