Fig. 4. Electron microscopy of in vivo biofilms.
Biofilm formation on central venous catheters inserted into rats and inoculated with C. parapsilosis (wild type strain CLIB214) and a bcr1 deletion (CDb71) strain. The catheters were removed after 24 hrs and visualized using scanning electron microscopy at two different magnifications. The wild type strain formed a thick biofilm, whereas the bcr1 deletion strain displayed impaired biofilm formation, indicating the essential role of the transcription factor BCR1 in biofilm formation. [Reproduced with permission from Ding C, Vidanes GM, Maguire SL, Guida A, Synnott JM, Andes DR and Geraldine Butler. Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans. PLoS One. 2011;6(12):e28151].