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. 2013 Jun 13;8(6):e65979. doi: 10.1371/journal.pone.0065979

Figure 2. Alcian blue / alizarin red S double staining of the skeleton of 1-day-old PiT1 +/+ and PiT1 neo/neo mice.

Figure 2

(A) Whole skeleton staining of PiT1 +/+ and PiT1 neo/neo mice. (B) Magnification on the skull: the frontal (fr), parietal (pa) and occipital (oc) bones are not mineralized in PiT1 neo/neo 1-day-old mice. (C) PiT1 neo/neo 1-day-old pups present a significant delay of mineralization at the ribs (white arrow head) and the spine (black arrow head). (D) Magnification on the hind limbs: the tibia (tb) and the fibula (fb) of PiT1 neo/neo 1-day-old mice are not mineralized, and a retardation of mineralization is observed in the femur (fm).