Table 2. Specific enzyme activity of Cel5A_2R2 and Cel5A_2R2-CBM6 on various soluble and insoluble cellulosic substrates.
| Substrates | Specific enzyme activity (U/µmol) | ||
| Cel5A | Cel5A_2R2 | Cel5A_2R2-CBM6 | |
| (41.6 kDa) | (41.6 kDa) | (72.0 kDa) | |
| CMC | 615±35 | 574±35 | 600±40 |
| Barley β-glucan | 3,490±90 | 3,410±100 | 3,480±100 |
| PASC | 80±10 | 75±10 | 144±10a |
| Filter paper | 0.41±0.05 | 0.40±0.05 | 2.88±0.5a |
| Avicel | 0.21±0.05 | 0.20±0.05 | 1.44±0.5a |
| p-NPC | 98±10 | 95±10 | 90±10 |
| p-NPG | NDb | NDb | NDb |
a
Specific activity of Cel5A_2R2-CBM6 was statistically significant from wild type Cel5A and mutant Cel5A_2R2 at two-tailed P value is less than 0.0001.
b
ND represents “Not Detected”, indicating no enzyme activity.