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. 2013 Apr 13;19(7):511–520. doi: 10.1111/cns.12098

Figure 3.

Figure 3

Zinc‐induced overexpression of HIF‐1α in astrocytes was involved with zinc‐induced cytotoxicity. siRNA‐HIF was used to downregulate the expression of HIF‐1α in C8‐D1A astrocytes before treatment with 100 μM zinc chloride and exposure to hypoxia. Control siRNA‐A, a nontargeting 20–25 nt siRNA, was used as a negative control. (A) The expression of HIF‐1α was determined by Western blot. (B) The effect of HIF‐1α on zinc‐induced cell death was measured by Cytotox 96 nonradioactive cytotoxicity assay kit. The experiments were repeated four times (n = 4). Data were presented as means ± SE. *P < 0.05. Mock: cells untreated with siRNA; siRNA‐A: cells were transfected with control siRNA before hypoxia/normoxia treatment; siRNA‐HIF: cells were transfected with HIF‐1α siRNA before hypoxia/normoxia treatment; siRNA‐A + zinc: cells were first transfected with control siRNA and then treated with hypoxia/normoxia in the presence of zinc; siRNA‐HIF + zinc: cells were first transfected with HIF siRNA and then treated with hypoxia/normoxia in the presence of zinc.