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. 2013 Jun 13;8(6):e65793. doi: 10.1371/journal.pone.0065793

Figure 2. Distribution and properties of small cryptic plasmids.

Figure 2

(A) Illustration of the content of small cryptic plasmids in the isolates. Circles represent plasmids and the plasmid size is shown on the y-axis in kilobases and the x-axis shows the sample ECO- identifier. (B) Neighbour-joining tree of small cryptic plasmids indicating their sequence-based relationships. Sequences were aligned with ClustalW v.2.1 and the tree was created with MEGA v.5 (default settings) [35]. Scale bar = substitutions per site. Support values are indicated at branches. (C and D) Sequence similarity blocks between small cryptic plasmids (violet stripes). Arrows indicate ORFs longer than 300 bp. Similarity blocks were identified with Mauve v.2.3.1 [36], and plotted with genoPlotR v.0.8.1. [37]. ARP = putative aminoglycoside resistance protein; T2DM = putative type II DNA- methyltransferase; RE = putative EcoVIII restriction endonuclease.