Figure 3. Immunoblot evaluation evaluating effects of NVP-AUY922 in vitro.
Immunoblot evaluation for cell lines with IC50 < 5 nM and IC100 < 40 nM (A), IC50 > 5 nM and IC100 < 40 nM (B) and IC100 > 40 nM (C) are shown. Measured proteins include HSP70, pERK 1/2 (Thr202/Tyr204), total ERK1/ERK2, pAKT (Ser473/Ser308), total AKT, and Tubulin at baseline and after 30 minutes, 18 and 24 hours of treatment with 50 nM or 100 nM of NVP-AUY922 as indicated.