Figure 2.
DN-PKCε inhibits TLC-induced MRP2 retrieval. HuH-NTCP cell transfected with HA-tagged DN-PKCε were treated with TLC for 25 min or CPT-cAMP for 15 min followed by biotinylation of cell surface proteins and immunoblot analysis of HA, PKCε, biotinylated MRP2 (PM-MRP2) and biotinylated E-cadherin. Typical immunoblots are shown in the upper panel and results of densitometric analysis (Mean±SEM, n=4) are shown in the bar graph. *Significantly different from respective control (CON) and #significantly different from respective empty vector (EV) values.