Figure 3. Inhibition of the activity of full-length PA endonuclease by the fullerene derivatives.

(A) Schematic of the constructed plasmid, baculovirus expression, and purification of full-length PA protein. (B) Purification of full-length PA protein using a HiTrap-Q column. The numbers indicate the fractions. The arrow indicates full-length PA protein. (C) The effects of the various fullerene derivatives on the endonuclease activity of full-length PA protein of influenza A RNA polymerase were tested. Recombinant full-length PA protein was added to each reaction at a concentration of 0.25 µg/100 µL. A zero control (i.e., no PA protein added) was also assayed. The fullerene derivatives were added at a dose of 10 µM and M13 mp18 was used as the substrate.