Table 4. List of primers used in this study for the construction of fliC, rcsB and lon mutants.

Name*	Primer 5′-3′
fliC_44F	GTCGGTGAATCAATCGCCGGATTAACGCAGTAAAGAGAGGACGT
fliC_44R	AGTCATTAATACAAACAGCCTGTCGCTGTTGACCCAGAATAACC
rcsB_44F	ATGAACAATATGAACGTAATTATTGCCGATGACCACCCGATTGT
rcsB_44R	TTATTCTTTGTCTGTCGGACTCAGGGTGACAGAAGAGAGATAGT
lon_51F	CAGCTATACTATCTGATTACCTGGCGGACACTAAACTAAGAGAGAGCTCTT
lon_50R	CGAAATAGCCTGCCAGCCCTGTTTTTATTAGCGCTATTTGCGCGAGGTCA
fliC_FCTR	TGGCGAGATATTTTTTAACC
fliC_RCTR	AGTAGTTAAGCGCGTTATCG
rcsB_FCTR	GGCTATTATGCGCTATTTGT
rcsB_RCTR	ATATTGTTCTGAGCGATGTG
lon_FCTR	GCAGGCTTCTGGCGAATAAT
lon_RCTR	CGACCGCGCAGCAGTTATAT

^*For primers used for the amplification of pKD3 or pKD4, only the gene specific overhangs are shown. „CTR“ primers, either Forward (F) or Reverse (R) were used for the verification of the final contructs.