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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Addict Biol. 2013 Mar 6;19(5):770–780. doi: 10.1111/adb.12040

Figure 3.

Figure 3

Photomicrographs of BrdU labeling from (a) WT-water only mouse and (b) WT-2BC mouse. Arrowhead points to BrdU clusters in the subgranular zone of the DG. Scale bar in (b) is 100 μm, applies to (a–b). (c–d) Quantitative analysis (cell counts) of 2-h BrdU-labeled S-phase progenitors (c) and Ki-67 proliferating cells (d) in the DG from the second group of mice (water only: WT, n = 4; Het, n = 4; KO, n = 4; 2BC: WT, n = 6; Het, n = 9; KO, n = 5). Post hoc analysis revealed significant differences between water-and ethanol-drinking mice of a given genotype (*, p < 0.05; **, p < 0.01). (e) Linear regression analysis of the effect of chronic binge drinking on progenitor proliferation in the DG. The total number of Ki-67-labeled cells in the hippocampus is plotted against the average ethanol intake over the last five drinking sessions for each mouse from the first and second groups.