Table 1. Mean Ct values and gene stability values provided by geNorm (M) and NormFinder (Sv), and Pearson correlation coefficient (rI) with calculated index from BestKeeper.
Gene | Uterus | Ovary | ||||||
Ct | GeNorm | NormFinder | BestKeeper | Ct | GeNorm | NormFinder | BestKeeper | |
(M) | (Sv) | (rI) | (M) | (Sv) | (rI) | |||
ACTB | 18.35 | 1.271 | 0.510 | 0.860 | 20.77 | 1.198 | 0.499 | 0.955 |
B2M | 18.69 | 1.578 | 0.541 | 0.565 | 18.82 | 0.897 | 0.384 | 0.878 |
GNB2L1 | 21.52 | 0.972 | 0.307 | 0.909 | 21.78 | 0.774 | 0.313 | 0.914 |
HMBS | 27.23 | 1.597 | 0.673 | 0.463 | 27.47 | 0.866 | 0.351 | 0.874 |
HPRT1 | 26.54 | 1.088 | 0.275 | 0.778 | 26.03 | 0.729 | 0.219 | 0.957 |
RPL32 | 22.26 | 1.003 | 0.321 | 0.904 | 22.34 | 0.788 | 0.311 | 0.913 |
SDHA | 24.61 | 0.965 | 0.276 | 0.895 | 23.98 | 0.869 | 0.368 | 0.889 |
TBP | 24.65 | 0.875 | 0.159 | 0.968 | 24.91 | 0.675 | 0.164 | 0.979 |
UBC | 20.75 | 0.944 | 0.309 | 0.918 | 21.33 | 0.907 | 0.457 | 0.881 |
YWHAZ | 21.48 | 1.101 | 0.263 | 0.906 | 21.67 | 0.869 | 0.421 | 0.990 |
The two most stable genes in each analysis are indicated in bold.