Table 4. Primers used in the real time quantitative PCR assay.
Primer Name | Sequence 5′→3′ | NCBI Sequence | Exons | Size |
ACTB_F | CACGCCATCCTGCGTCTGGA | XM_003357928.1 | 4,5 | 100 bp |
ACTB_R | AGCACCGTGTTGGCGTAGAG | |||
B2M_F | TCGGGCTGCTCTCACTGTCT | NM_213978 | 1, 2 | 69 bp |
B2M_R | GGCGTGAGTAAACCTGAACCTT | |||
GNB2L1_F | CCCGAGATAAAACCATCAAGCT | NM_214332 | 4, 5 | 93 bp |
GNB2L1_R | CGGACACAAGACACCCACTCT | |||
HMBS_F | AGGATGGGCAACTCTACCTG | NM_001097412.1 | 15,16 | 83 bp |
HMBS_R | GATGGTGGCCTGCATAGTCT | |||
HPRT1_F | AAGATGGTCAAGGTTGCAAGCT | NM_001032376.2 | 7, 8, 9 | 82 bp |
HPRT1_R | ATTTCAAATCCAACAAAGTCTGGTCTA | |||
RPL32_F | CACCAGTCAGACCGATATGTCAA | NM_001001636 | 1, 2 | 70 bp |
RPL32_R | CGCACCCTGTTGTCAATGC | |||
SDHA_F | CTACAAGGGGCAGGTTCTGA | XM_003362140.1 | 19, 20 | 141 bp |
SDHA_R | AAGACAACGAGGTCCAGGAG | |||
TBP_F | AACAGTTCAGTAGTTATGAGCCAGA | DQ178129.1 | 8, 9, 10 | 153 bp |
TBP_R | AGATGTTCTCAAACGCTTCG | |||
UBC_F | GCATTGTTGGCGGTTTCG | NM_001105309.1 | 2,3 | 64 bp |
UBC_R | AGACGCTGTGAAGCCAATCA | |||
YWHAZ_F | TGATGATAAGAAAGGGATTGTGG | XM_001927228.4 | 3,4 | 134 bp |
YWHAZ_R | CTCATAATAGAACACAGAGA |