Skip to main content
. 2013 Jun 13;8(6):e66113. doi: 10.1371/journal.pone.0066113

Figure 5. Cai and pHi depend on the presence of external HCO3–.

Figure 5

A. Observation 80 mins after fertilization. Eggs develop in ASW (Control) or are transferred 20 mins after fertilization into ASW deprived of Ca (0Ca), HCO3 − (0HCO3) or of Na+ and Ca++ (0Na0Ca). B. Time course after fertilization of pHi (a) and Cai (b) in ASW, 0Na and 0Na0Ca in the presence or not of HCO3 −. Examples of results obtained in absence of HCO3-, i.e. in 0HCO3 (1), 0Na0HCO3 (2) and 0Na0Ca0HC03 (3), are depicted in different grey colours and representative of n determinations (grey number in brackets). In each condition, one typical control experiment performed with the same population of eggs in the presence of HCO3 −, i.e. in ASW (1), 0Na (2) and 0Na0Ca (3) is shown in black curve and representative of n determinations (black number in brackets).