Table 1. Oligonucleotide primers/probes used for this study.
qPCR primers/probe | Sequence (5′- 3′) | Target | Annealing (°C) | Reference |
CFB555f | CCGGAWTYATTGGGTTTAAAGGG | Bacteroidetes | 60 | 56 |
CFB968r | GGTAAGGTTCCTCGCGTA | |||
Fuso-F | KGGGCTCAACMCMGTATTGCGT | Fusobacteria | 51 | 26 |
Fuso-R | TCGCGTTAGCTTGGGCGCTG | |||
341-F | CCTACGGGAGGCAGCAGT | Universal Bacteria | 59 | 57 |
518-R | ATTACCGCGGCTGCTGG | |||
EntF | CCCTTATTGTTAGTTGCCATCATT | Enterococcus | 61 | 58 |
EntR | ACTCGTTGTACTTCCCATTGT | |||
EcolRT_F | GTTAATACCTTTGCTCATTGA | E. coli | 55 | 59 |
EcolRT R | ACCAGGGTATCTAATCCTGTT |