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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Genesis. 2012 Aug 20;50(12):899–907. doi: 10.1002/dvg.22046

Figure 2. The Megsin-Cre transgene has expression specifically in the skin.

Figure 2

Whole-mount β-galactosidase assay (A–B) and X-gal analysis on skin cryostat section (C) revealed the occurrence of Cre-mediated loxP recombination in the hair follicles and epidermis. A: E14.5 embryos. B: skin of P3 transgenic mouse. C: skin section from P3 transgenic mouse. Immunohistochemical analysis indicated Megsin was expressed in all epidermal layers (D-L). D-F: YFP colocalized with E-cadherin staining in the skin epithelium, G-I: YFP colocalized with K10 staining in the suprabasal layers of the epidermis and other cornified stratified epithelia, J-L: YFP colocalized with K14 staining in the basal layer of the epidermis. Scale bar: 100 μm.