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. Author manuscript; available in PMC: 2013 Jun 13.
Published in final edited form as: Small. 2011 Sep 16;7(22):3158–3162. doi: 10.1002/smll.201101018

Figure 1.

Figure 1

Human protein kinase C epsilon (PRKCε) expression as a function of nanoparticle-conjugate treatment in PC-3 cells. (A) The construction of the pMIR-REPORT™ vector. The duplex formation demonstrates the partially complementary binding between miR-205 and PRKCε mRNA at position 2732–2738 of PRKCε 3′-UTR. (B) Bar graph shows that firefly luciferase activity is suppressed by transfection of mimic miR-205 Au NPs when compared with the non-targeting Au NPs. Commercially available mimic miR-205, transfected using DharmaFECT™, shows 12% reduction in firefly luciferase activity. The error bars indicate the standard deviation (SD) from three different experiments. (C) Immunoblotting of PC-3 cell lysates for PRKCε, showing an over 50% decrease in PRKCε expression in PC-3 cells following transfection with mimic miR-205 Au NPs. GAPDH serves as the loading control. The relative protein expression level was quantified by ImageJ.