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. 2013 Jun 13;8(6):e66501. doi: 10.1371/journal.pone.0066501

Figure 1. NLGP modulates immunosuppressive cytokine milieu within TME.

Figure 1

A. Sarcoma 180 tumor tissues (100 mg) harvested from Swiss albino mice was lysed by freeze-thaw technique in 1 ml PBS supplemented with a cocktail of protease inhibitors. Tumor tissue lysates, representing TME from either PBS or NLGP treated mice (n = 6 in each case) were assessed for IFNγ, IL-2, IL-12, IL-6 and IL-10 by ELISA. Cytokines were quantitated as pg/mg of tumor tissue ± SE. *p<0.001, **p<0.05, in comparison to PBS treated tumor on day 15 and 20. B.1. Total RNA was isolated from tumor of PBS and NLGP treated mice (n = 6 in each case) to analyze genes of IFNγ, IL-2, IL-12, IL-6 and IL-10 by RT-PCR. B.2. Densitometric analysis was performed in each case, ▪p<0.01. C.1. STAT3 and pSTAT3 levels were studied in total protein isolated from PBS and NLGP tumors (n = 3, in each case) by Western blot analysis, C.2. and data from three individual observations was analyzed by densitometric scanning, in comparison to PBS treated tumor on day 15 and 20, ▪p<0.01.