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Total RNA was isolated from tumors of PBS and NLGP treated mice. cDNA was prepared from RNA and PCR was performed for different chemokine related genes, A.1. ccr5, ccl3, ccl4, ccl5, ccl8; B.1. cxcr3, cxcl9, cxcl10; C.1. cxcr4, cxcl12. A.2, B.2, C.2. Densitometric analysis was performed in each case.
