Table 2. Primer sequences of various genes of cytokine, growth factors etc.
| Name | Primer sequences (5′-3′) | Product size |
| IFNγ-forward | ACTGGCAAAAGGATGGTGAC | 237 bp |
| IFNγ-reverse | TGAGCTCATTGAATGCTTGG | |
| IL-2- forward | GCAGGCCACAGAATTGAAAG | 207 bp |
| IL-2- reverse | TCCACCACAGTTGCTGACTC | |
| IL-12- forward | CCTGCATCTAGAGGCTGTCC | 243 bp |
| IL-12- reverse | CATCTTCTTCAGGCGTGTCA | |
| IL-6- forward | TTCCATCCAGTTGCCTTCTT | 199 bp |
| IL-6- reverse | CAGAATTGCCATTGCACAAC | |
| IL-10- forward | CCAAGCCTTATCGGAAATGA | 162 bp |
| IL-10- reverse | TTTTCACAGGGGAGAAAATCG | |
| TGFβ-forward | TGCGCTTGCAGAGATTAAAA | 197 bp |
| TGFβ-reverse | GCTGAATCGAAAGCCCTGTA | |
| VEGF-forward | GGACCCTGGCTTTACTGCTG | 201 bp |
| VEGF-reverse | CACAGGACGGCTTGAAGATG | |
| HIF1α-forward | TCAAGTCAGCAACGTGGAAG | 198 bp |
| HIF1α-reverse | TATCGAGGCTGTGTCGACTG | |
| VEGFR2-forward | ACAGACAGTGGGATGGTCC | 271 bp |
| VEGFR2-reverse | AAACAGGAGGTGAGCGCAG | |
| VEGFR1-forward | CCAACTACCTCAAGAGCAAAC | 315 bp |
| VEGFR1-reverse | CCAGGTCCCGATGAATGCAC | |
| IDO1-forward | TGAAAAGCTGCCCACACTGA | 300 bp |
| IDO1-reverse | CAGTCCCCACCAGGAAATGA | |
| CTLA4 -forward | TCAGTGGTGTTGGCTAGCAG | 228 bp |
| CTLA4-reverse | CAGTCCTTGGATGGTGAGGT | |
| Perforin-forward | GATGTGAACCCTAGGCCAGA | 161 bp. |
| Perforin-reverse | GGTTTTTGTACCAGGCGAAA | |
| Granzyme B-forward | TCGACCCTACATGGCCTTAC | 198 bp. |
| Granzyme B-reverse | TGGGGAATGCATTTTACCAT |