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. 2013 Jun 13;8(6):e65628. doi: 10.1371/journal.pone.0065628

Figure 3. Slx5 binding to centromeres is largely kinetochore-dependent.

Figure 3

(A) Live cell microscopy of cells coexpressing Slx5-GFP and kinetochore protein Nnf1-mCherry. Right-hand panel shows a magnification of two nuclei, indicated in the merged image. Scale bars, 5 µm. (B) ChIP-qPCR of Slx5-GFP and Slx8-GFP in wt, slx5Δ or slx8Δ strains. Binding ratios (BR) at CEN1 and CEN2 are represented as enrichment over the control gene POL1 (± s.d., n = 4), based on two independent biological replicate experiments. (C–D) ChIP-qPCR of Slx5-GFP in wt and ndc10-1 strains at permissive (25°C) and nonpermissive (37°C) temperatures. Data is represented as enrichment at CEN2 over POL1 (Slx5-GFP and Slx5-GFP ndc10-1: ± s.d., n = 2) (wt and ndc10-1: n = 1, no enrichment).