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. 1994 Jan;1(1):89–94. doi: 10.1128/cdli.1.1.89-94.1994

Neutralizing antibody immune response in children with primary and secondary rotavirus infections.

C F Arias 1, S López 1, J D Mascarenhas 1, P Romero 1, P Cano 1, Y B Gabbay 1, R B de Freitas 1, A C Linhares 1
PMCID: PMC368202  PMID: 7496929

Abstract

We have characterized the neutralizing antibody immune response to six human rotavirus serotypes (G1 to G4, G8, and G9) in Brazilian children with primary and secondary rotavirus infections and correlated the response with the G serotype of the infecting rotavirus strain. Twenty-five children were studied: 17 had a single rotavirus infection, 4 were reinfected once, and 4 experienced three infections. Two of the reinfections were by non-group A rotaviruses. Among the 25 primary infections, we observed homotypic as well as heterotypic responses; the serotype G1 viruses, which accounted for 13 of these infections, induced mostly a homotypic response, while infections by serotype G2 and G4 viruses induced, in addition to the homotypic, a heterotypic response directed primarily to serotype G1. Two of the primary infections induced heterotypic antibodies to 69M, a serotype G8 virus that by RNA electrophoresis analysis was found not to circulate in the population during the time of the study. The specificity of the neutralizing antibody immune response induced by a virus of a given serotype was the same in primary as well as secondary infections. These results indicate that the heterotypic immune response induced in a primary rotavirus infection is an intrinsic property of the virus strain, and although there seem to be general patterns of serotype-specific seroconversion, these may vary from serotype to serotype and from strain to strain within a serotype.

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Selected References

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  1. Arias C. F., Lizano M., López S. Synthesis in Escherichia coli and immunological characterization of a polypeptide containing the cleavage sites associated with trypsin enhancement of rotavirus SA11 infectivity. J Gen Virol. 1987 Mar;68(Pt 3):633–642. doi: 10.1099/0022-1317-68-3-633. [DOI] [PubMed] [Google Scholar]
  2. Beards G., Xu L., Ballard A., Desselberger U., McCrae M. A. A serotype 10 human rotavirus. J Clin Microbiol. 1992 Jun;30(6):1432–1435. doi: 10.1128/jcm.30.6.1432-1435.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Browning G. F., Chalmers R. M., Fitzgerald T. A., Snodgrass D. R. Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype. J Gen Virol. 1991 May;72(Pt 5):1059–1064. doi: 10.1099/0022-1317-72-5-1059. [DOI] [PubMed] [Google Scholar]
  4. Browning G. F., Fitzgerald T. A., Chalmers R. M., Snodgrass D. R. A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate FI23. J Clin Microbiol. 1991 Sep;29(9):2043–2046. doi: 10.1128/jcm.29.9.2043-2046.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brüssow H., Clark H. F., Sidoti J. Prevalence of serum neutralizing antibody to serotype 9 rotavirus WI61 in children from South America and central Europe. J Clin Microbiol. 1991 Jan;29(1):208–211. doi: 10.1128/jcm.29.1.208-211.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brüssow H., Sidoti J. Antibody to serotype 8 rotavirus in Ecuadorian and German children. Epidemiol Infect. 1991 Apr;106(2):415–420. doi: 10.1017/s0950268800048561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Brüssow H., Sidoti J., Barclay D., Sotek J., Dirren H., Freire W. B. Prevalence and serotype specificity of rotavirus antibodies in different age groups of Ecuadorian infants. J Infect Dis. 1990 Sep;162(3):615–620. doi: 10.1093/infdis/162.3.615. [DOI] [PubMed] [Google Scholar]
  8. Brüssow H., Werchau H., Lerner L., Mietens C., Liedtke W., Sidoti J., Sotek J. Seroconversion patterns to four human rotavirus serotypes in hospitalized infants with acute rotavirus gastroenteritis. J Infect Dis. 1988 Sep;158(3):588–595. doi: 10.1093/infdis/158.3.588. [DOI] [PubMed] [Google Scholar]
  9. Chen D. Y., Estes M. K., Ramig R. F. Specific interactions between rotavirus outer capsid proteins VP4 and VP7 determine expression of a cross-reactive, neutralizing VP4-specific epitope. J Virol. 1992 Jan;66(1):432–439. doi: 10.1128/jvi.66.1.432-439.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chiba S., Yokoyama T., Nakata S., Morita Y., Urasawa T., Taniguchi K., Urasawa S., Nakao T. Protective effect of naturally acquired homotypic and heterotypic rotavirus antibodies. Lancet. 1986 Aug 23;2(8504):417–421. doi: 10.1016/s0140-6736(86)92133-1. [DOI] [PubMed] [Google Scholar]
  11. Clark H. F., Dolan K. T., Horton-Slight P., Palmer J., Plotkin S. A. Diverse serologic response to rotavirus infection of infants in a single epidemic. Pediatr Infect Dis. 1985 Nov-Dec;4(6):626–631. doi: 10.1097/00006454-198511000-00006. [DOI] [PubMed] [Google Scholar]
  12. Clark H. F., Hoshino Y., Bell L. M., Groff J., Hess G., Bachman P., Offit P. A. Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol. 1987 Sep;25(9):1757–1762. doi: 10.1128/jcm.25.9.1757-1762.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Estes M. K., Cohen J. Rotavirus gene structure and function. Microbiol Rev. 1989 Dec;53(4):410–449. doi: 10.1128/mr.53.4.410-449.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Flores J., Midthun K., Hoshino Y., Green K., Gorziglia M., Kapikian A. Z., Chanock R. M. Conservation of the fourth gene among rotaviruses recovered from asymptomatic newborn infants and its possible role in attenuation. J Virol. 1986 Dec;60(3):972–979. doi: 10.1128/jvi.60.3.972-979.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gerna G., Battaglia M., Milenesi G., Passarani N., Percivalle E., Cattaneo E. Serotyping of cell culture-adapted subgroup 2 human rotavirus strains by neutralization. Infect Immun. 1984 Feb;43(2):722–729. doi: 10.1128/iai.43.2.722-729.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Gerna G., Sarasini A., Parea M., Arista S., Miranda P., Brüssow H., Hoshino Y., Flores J. Isolation and characterization of two distinct human rotavirus strains with G6 specificity. J Clin Microbiol. 1992 Jan;30(1):9–16. doi: 10.1128/jcm.30.1.9-16.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Gerna G., Sarasini A., Torsellini M., Torre D., Parea M., Battaglia M. Group- and type-specific serologic response in infants and children with primary rotavirus infections and gastroenteritis caused by a strain of known serotype. J Infect Dis. 1990 Jun;161(6):1105–1111. doi: 10.1093/infdis/161.6.1105. [DOI] [PubMed] [Google Scholar]
  18. Gorziglia M., Larralde G., Kapikian A. Z., Chanock R. M. Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proc Natl Acad Sci U S A. 1990 Sep;87(18):7155–7159. doi: 10.1073/pnas.87.18.7155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Grimwood K., Lund J. C., Coulson B. S., Hudson I. L., Bishop R. F., Barnes G. L. Comparison of serum and mucosal antibody responses following severe acute rotavirus gastroenteritis in young children. J Clin Microbiol. 1988 Apr;26(4):732–738. doi: 10.1128/jcm.26.4.732-738.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hardy M. E., Gorziglia M., Woode G. N. Amino acid sequence analysis of bovine rotavirus B223 reveals a unique outer capsid protein VP4 and confirms a third bovine VP4 type. Virology. 1992 Nov;191(1):291–300. doi: 10.1016/0042-6822(92)90191-q. [DOI] [PubMed] [Google Scholar]
  21. Hoshino Y., Wyatt R. G., Greenberg H. B., Flores J., Kapikian A. Z. Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization. J Infect Dis. 1984 May;149(5):694–702. doi: 10.1093/infdis/149.5.694. [DOI] [PubMed] [Google Scholar]
  22. Linhares A. C., Gabbay Y. B., Freitas R. B., da Rosa E. S., Mascarenhas J. D., Loureiro E. C. Longitudinal study of rotavirus infections among children from Belém, Brazil. Epidemiol Infect. 1989 Feb;102(1):129–145. doi: 10.1017/s0950268800029769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Linhares A. C., Gabbay Y. B., Mascarenhas J. D., Freitas R. B., Flewett T. H., Beards G. M. Epidemiology of rotavirus subgroups and serotypes in Belem, Brazil: a three-year study. Ann Inst Pasteur Virol. 1988 Jan-Mar;139(1):89–99. doi: 10.1016/s0769-2617(88)80009-1. [DOI] [PubMed] [Google Scholar]
  24. Losonsky G. A., Reymann M. The immune response in primary asymptomatic and symptomatic rotavirus infection in newborn infants. J Infect Dis. 1990 Feb;161(2):330–332. doi: 10.1093/infdis/161.2.330. [DOI] [PubMed] [Google Scholar]
  25. Matson D. O., O'Ryan M. L., Pickering L. K., Chiba S., Nakata S., Raj P., Estes M. K. Characterization of serum antibody responses to natural rotavirus infections in children by VP7-specific epitope-blocking assays. J Clin Microbiol. 1992 May;30(5):1056–1061. doi: 10.1128/jcm.30.5.1056-1061.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Matsui S. M., Mackow E. R., Greenberg H. B. Molecular determinant of rotavirus neutralization and protection. Adv Virus Res. 1989;36:181–214. doi: 10.1016/s0065-3527(08)60585-0. [DOI] [PubMed] [Google Scholar]
  27. Matsuno S., Hasegawa A., Mukoyama A., Inouye S. A candidate for a new serotype of human rotavirus. J Virol. 1985 May;54(2):623–624. doi: 10.1128/jvi.54.2.623-624.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Nakata S., Estes M. K., Graham D. Y., Loosle R., Tao H., Wang S. H., Saif L. J., Melnick J. L. Antigenic characterization and ELISA detection of adult diarrhea rotaviruses. J Infect Dis. 1986 Sep;154(3):448–455. doi: 10.1093/infdis/154.3.448. [DOI] [PubMed] [Google Scholar]
  29. Offit P. A., Hoffenberg E. J., Santos N., Gouvea V. Rotavirus-specific humoral and cellular immune response after primary, symptomatic infection. J Infect Dis. 1993 Jun;167(6):1436–1440. doi: 10.1093/infdis/167.6.1436. [DOI] [PubMed] [Google Scholar]
  30. Offit P. A., Shaw R. D., Greenberg H. B. Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins vp3 and vp7. J Virol. 1986 May;58(2):700–703. doi: 10.1128/jvi.58.2.700-703.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Padilla-Noriega L., Arias C. F., López S., Puerto F., Snodgrass D. R., Taniguchi K., Greenberg H. B. Diversity of rotavirus serotypes in Mexican infants with gastroenteritis. J Clin Microbiol. 1990 Jun;28(6):1114–1119. doi: 10.1128/jcm.28.6.1114-1119.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Puerto F. I., Padilla-Noriega L., Zamora-Chávez A., Briceño A., Puerto M., Arias C. F. Prevalent patterns of serotype-specific seroconversion in Mexican children infected with rotavirus. J Clin Microbiol. 1987 May;25(5):960–963. doi: 10.1128/jcm.25.5.960-963.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Qian Y., Green K. Y. Human rotavirus strain 69M has a unique VP4 as determined by amino acid sequence analysis. Virology. 1991 May;182(1):407–412. doi: 10.1016/0042-6822(91)90691-4. [DOI] [PubMed] [Google Scholar]
  34. Taniguchi K., Urasawa S., Urasawa T. Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. J Gen Virol. 1985 May;66(Pt 5):1045–1053. doi: 10.1099/0022-1317-66-5-1045. [DOI] [PubMed] [Google Scholar]
  35. Taniguchi K., Urasawa T., Kobayashi N., Ahmed M. U., Adachi N., Chiba S., Urasawa S. Antibody response to serotype-specific and cross-reactive neutralization epitopes on VP4 and VP7 after rotavirus infection or vaccination. J Clin Microbiol. 1991 Mar;29(3):483–487. doi: 10.1128/jcm.29.3.483-487.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Taniguchi K., Urasawa T., Kobayashi N., Gorziglia M., Urasawa S. Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern: implication for new G serotype specificity. J Virol. 1990 Nov;64(11):5640–5644. doi: 10.1128/jvi.64.11.5640-5644.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Urasawa S., Urasawa T., Taniguchi K., Chiba S. Serotype determination of human rotavirus isolates and antibody prevalence in pediatric population in Hokkaido, Japan. Arch Virol. 1984;81(1-2):1–12. doi: 10.1007/BF01309292. [DOI] [PubMed] [Google Scholar]
  38. Zheng B. J., Han S. X., Yan Y. K., Liang X. R., Ma G. Z., Yang Y., Ng M. H. Development of neutralizing antibodies and group A common antibodies against natural infections with human rotavirus. J Clin Microbiol. 1988 Aug;26(8):1506–1512. doi: 10.1128/jcm.26.8.1506-1512.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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