Table 2.

PCR amplification conditions in this study

S1/S2	10 min at 94 °C; 40 s at 94 °C, 40 s at 49 °C, and 50 s at 72 °C for 35 cycles; 5 min at 72 °C
5R1/AAP	10 min at 94 °C; 40 s at 94 °C, 40 s at 51 °C, and 1 min at 72 °C for 28 cycles; 5 min at 72 °C
5R2/AUAP	10 min at 94 °C; 40 s at 94 °C, 40 s at 48 °C, and 40 s at 72 °C for 35 cycles; 5 min at 72 °C
3R1/B26	10 min at 94 °C; 40 s at 94 °C, 40 s at 49 °C, and 1 min at 72 °C for 28 cycles; 5 min at 72 °C
3R2/B25	10 min at 94 °C; 40 s at 94 °C, 40 s at 52 °C, and 40 s at 72 °C for 35 cycles; 5 min at 72 °C
QC1/QC2	10 min at 94 °C; 40 s at 94 °C, 40 s at 45 °C, and 1 min at 72 °C for 35 cycles; 5 min at 72 °C
SN1/SN2	10 min at 94 °C; 40 s at 94 °C, 40 s at 43 °C, and 1 min 30 s at 72 °C for 35 cycles; 5 min at 72 °C
SN3/SN4	10 min at 94 °C; 40 s at 94 °C, 40 s at 51 °C, and 1 min 30 s at 72 °C for 35 cycles; 5 min at 72 °C
YH-F/YH-R	10 min at 94 °C; 40 s at 94 °C, 40 s at 50 °C, and 40 s at 72 °C for 35 cycles; 5 min at 72 °C