Figure 5.

Effects of diazepam, osemozotan (upper panels), and LY379268 (lower panels) on encounter-induced behavioral changes in male isolation-reared mice. A male isolation-reared mouse was placed into the large compartment of a novel clear Plexiglas cage divided by a mesh partition. After a 3-h habituation period, the resident mice were intraperitoneally injected with vehicle, diazepam (1 mg/kg), or osemozotan (0.3 mg/kg) 30 min before the encounter with an unfamiliar male intruder for 20 min. Saline or LY379268 (1 mg/kg) was intraperitoneally injected 60 min before the encounter. (a, c) Representative locomotor paths of the resident mice during the 20-min encounters are shown. (b, d) The locomotor activity of and total time spent in the gray zone by the resident mice and the total time the resident and intruder mice spent interacting were analyzed according to the description in the Materials and Methods. The data are expressed as the mean±SEM of 10 mice/group. *p<0.05, **p<0.01, compared with the vehicle or saline-treated mice.