Figure 6.

Effects of diazepam, osemozotan (left panels), and LY379268 (right panels) on encounter-induced c-Fos expression in the prefrontal cortex (a), nucleus accumbens shell (b), dorsal raphe nucleus (c), and ventral tegmental area (d) of male group- and isolation-reared mice. A mouse was placed into the large compartment of a novel clear Plexiglas cage divided by a mesh partition. After a 3-h habituation period, the resident mice were intraperitoneally injected with vehicle, diazepam (1 mg/kg), or osemozotan (0.3 mg/kg) 30 min before the encounter with an unfamiliar male intruder for 20 min. Saline or LY379268 (1 mg/kg) was intraperitoneally injected 60 min before the encounter. Immunohistochemical localization of the neuronal-activity-marker c-Fos was determined 2 h after the encounter. The data are expressed as the mean±SEM of 6 mice/group. *p<0.05, **p<0.01, compared with the vehicle or saline-treated group-reared mice. ^†p<0.05, ^††p<0.01, compared with the vehicle or saline-treated isolation-reared mice.