Figure 1.

TIGAR-Deficient Mice

(A) Western blot analysis of the indicated tissues from WT and TIGAR-deficient animals.

(B) Mendelian ratio from TIGAR^+/− matings.

(C) Western blot analysis of baby mouse kidney (BMK) cultures from wild-type (WT), TIGAR^−/− (KO), and KO cultures transfected with human TIGAR construct.

(D) Level of fructose-2,6-bisphosphate in WT, KO, and KO BMK cell cultures transfected with TIGAR construct (KO + TIGAR). ^∗p < 0.05 compared to WT; ^∗∗p < 0.05 compared to KO.

(E) Ratio of oxidized and reduced glutathione (GSH/GSSG) of WT, KO, and KO + TIGAR BMK cells. ^∗p < 0.05 compared to WT; ^∗∗p < 0.05 compared to KO.

(F) Cell death as measured by PI exclusion of WT, KO, and KO + TIGAR BMK cells after hydrogen peroxide treatment. ^∗p < 0.05 compared to WT; ^∗∗p < 0.05 compared to KO.

(G and H) Untreated small intestine (G) and colon (H) from WT and KO animals. Top: hematoxylin and eosin staining (H&E); middle: Ki67; bottom: TIGAR staining. Scale bar, 200 μm. Data are represented as mean ± SEM (n = at least 3).

See also Figure S1.