Figure 2.

TIGAR-Deficient Mice Have Reduced Regenerative Capacity in the Intestinal Crypt after 14 Gy Whole-Body IR

(A) Small intestine from WT and TIGAR^−/− (KO) animals 72 hr after 14 Gy IR. Bar = 200 μm.

(B) Number of crypts per millimeter (left) and size of crypts (right) 72 hr after 14 Gy IR. ^∗p < 0.05 compared to WT.

(C) Ki67 staining of WT and KO intestines 6 and 72 hr after 14 Gy IR. Bar = 200 μm.

(D) Quantification of Ki67⁺ cells at the indicated times after 14 Gy IR. ^∗p < 0.05 compared to WT.

(E) Apoptosis in the small intestine 6 hr after 14 Gy IR. Asterisks denote cells with apoptotic nuclear morphology.

(F) Number of apoptotic cells in the crypts 6 hr after 14 Gy IR. ^∗p < 0.05 compared to WT.

(G) TIGAR staining of WT animals before IR and 24 and 72 hr after 14 Gy IR. Scale bar, 200 μm. Panels show details of crypt structures indicated in the box.

(H) Malondialdehyde (MDA) staining of WT and KO animals before and 24 hr after 14 Gy IR. Scale bar, 200 μm. Data are represented as mean ± SEM (n = at least 3).

See also Figure S2.