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The level of 14-3-3ς in growth cones decreases with age. (A) Representative 14-3-3ς IF staining in growth cones cultured for 8, 24, and 48 h (scale bars = 5 μm). (B) Quantitation of mean 14-3-3ς IF intensity/unit area in growth cones cultured 8, 24, 48 h (n = no. of growth cones; three replicates; ** = p<0.01; one-way ANOVA with Bonferroni post-test).
