Skip to main content
. Author manuscript; available in PMC: 2013 Jun 14.
Published in final edited form as: Gastroenterology. 2012 Mar 6;142(7):1504–15.e3. doi: 10.1053/j.gastro.2012.02.050

Figure 1.

Figure 1

Aspirin inhibits mTOR and activates AMPK in CRC cells. Aspirin-treated CRC whole-cell lysates were immunoblotted. Aspirin inhibits phosphorylation of (A) S6K1, S6 ribosomal protein (S6), and (B) 4E-BP1 in CRC cells. (C) Aspirin induces phosphorylation of AMPK and its substrate ACC in CRC cells. Imagequant software (GE Healthcare, Little Chalfont, UK) was used for densitometry, which is presented as the ratio of phosphorylated to total protein corrected for actin. Phenformin-treated CRC cells were used as controls. AMPK was immunoprecipitated from aspirin-treated whole-cell lysates and assayed with the AMARA peptide. (D) Aspirin increases AMPK activity in HCT116 cells (mean of 3 independent experiments).