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Peptide substrates constructed for this study: A) Cyclic, oxidized octamer; B) Acyclic, oxidized octamer; C) Acyclic hexamer in which Cys1 forms a mixed disulfide with the thiol of TNB, referred to as Pep-TNB in the text; and D) Cyclic octamer “switch” peptide in which Sec and Cys and have switched positions in the dyad. In all cases X = S or Se.
