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. Author manuscript; available in PMC: 2013 Jun 14.
Published in final edited form as: Biochemistry. 2008 Dec 2;47(48):12810–12821. doi: 10.1021/bi800951f

Table 1.

Kinetic Constants of Various TRs for the Reduction of Thioredoxin.

Enzyme-tetrapeptide Substrate kcat (min−1) Km (μM) kcat/Km (min−1•M−1)
rTR-GCUGa H. sapiens Trx 2500 3.3 7.6 ×108
rTR-GCCGa H. sapiens Trx 14.3 0.4 3.6 ×107
mTR-GCUGb E. coli Trx 2220 70 3.3 ×107
mTR-GCCGb E. coli Trx 4.1 49 8.4 ×104
CeTR2-GCCGc E. coli Trx 610 610 1 ×106
DmTR-SCCSd D. m. Trx 1320 7.1 1.9 ×108
DmTR-SCCSb E. coli Trx 300 170 1.7 ×106
AgTR-TCCSe A. g. Trx 924 8.5 1.13 ×108
a

Taken from (8).

b

Taken from (10).

c

Taken from (11).

d

Taken from (9).

e

Taken from (12).