Table 2.
Kinetic Constants for the Reduction of DTNB
| Enzyme | kcat (min−1) | Km (mM−1) | kcat/Km (min−1•mM−1) |
|---|---|---|---|
| mTR3-GCUGa | 1251 ± 71 | 0.45 ± 0.09 | 2720 |
| mTR3-Δ8 | 2905 ± 232 | 0.83 ± 0.27 | 3500 |
| DmTR-SCCSa | 157 ± 12 | 0.22 ± 0.07 | 713 |
| DmTR-Δ8 | 1294 ± 277 | 4.1 ± 2.1 | 316 |
| CeTR2-GCCGa | 134 ± 7 | 0.41 ± 0.09 | 327 |
| CeTR2-Δ8 | 152 ± 14 | 0.53 ± 0.19 | 287 |
Previously reported (10).