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. Author manuscript; available in PMC: 2013 Jun 14.
Published in final edited form as: J Immunol. 2009 Feb 15;182(4):2102–2112. doi: 10.4049/jimmunol.0802769

FIGURE 1.

FIGURE 1

Surface PD-L1 is expressed by DCs, LNSCs, and T cells, but not IECs. Expression of PD-L1 on DCs, LNSCs, IECs, polyclonal CD8+ T cells, and OT-I TCR transgenic T cells (gated on 7-aminoactinomycin D (7AAD)−CD45+CD11chigh, 7AAD−CD45−, 7AAD−CD45− UEA-I+, CD45+CD8+, and CD8+Vα2+ cells, respectively) was analyzed by flow cytometry. Overlays depict staining with anti-PD-L1 Ab for the indicated cell-type (open histogram) compared with isotype control (gray-filled histogram). For each cell type, tissues from n = 3–6 individual mice were analyzed and representative results are shown.