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. 2013 Jun 12;33(24):10048–10056. doi: 10.1523/JNEUROSCI.0217-13.2013

Figure 2.

Figure 2.

Alteration in molecular motor kinesins in twitcher sciatic nerves. Immunoblotting analyses for total KHC and KLC to test for changes in relative amounts of these motors 7- and 30-day (P7 and P30) spinal cords (a) and sciatic nerves (b) (n = 4/condition/group; actin used as loading control) revealed no significant differences in either KHC (c) and KLC (e) in spinal cord extracts from the mutants at either time point, although the P30 time point shows a trend of reduced amount of these motors (p > 0.05; t test unpaired). Levels of KHC (d) and KLC (f) differed significantly (p = 0.03, unpaired t test) in P30 twitcher sciatic nerves but were less affected in P7 mutant sciatic nerves. Immunoblotting analyses using monoclonal antibody 63-90, which detects changes in phosphorylation of KLC by GSK3β in 7- and 30-day-old (P7 and P30) spinal cords (g) and sciatic nerves (h) (n = 4/condition/group), revealed significant (p = 0.002–0.015, unpaired t test) differences in the level of dephosphorylated KLC (de-pKLC) in all twitcher samples except in P7 mutant sciatic nerves (i,j).