Skip to main content
. 2013 Jun 12;33(24):9920–9931. doi: 10.1523/JNEUROSCI.5482-12.2013

Figure 2.

Figure 2.

Temperature-dependent intrinsic firing of pyramidal neurons. A, B, Extracellular recordings of pyramidal neurons in acute hippocampal slices in physiologic ACSF (A) and in the presence of blockers of fast synaptic transmission (B). When the temperature (bottom traces) reached ∼38°C, cells started firing. The apparent increase in spike amplitude at high temperature is attributable to higher seal resistance (Fig. 1). C, Firing frequency in intact neurons plotted against bath temperature for cells recorded in ACSF (open symbols) and in the presence of kynurenic acid and picrotoxin (filled symbols). Bars above the plots show approximately the boundaries for hypothermia (white), normothermia (gray), and hyperthermia (black). D, Temperature-dependent firing was also detected in pyramidal cells in the entorhinal (EC; 35.7% of the cells, n = 14) and occipital (OC; 18.2% of the cells, n = 22) cortices. All the recordings in these areas were performed after blockade of fast synaptic transmission. E, Whole-cell recording from a pyramidal neuron in a hippocampal slice in ACSF while increasing the temperature (bottom trace) to 40°C. F, Similar recording in the presence of blockers of fast synaptic transmission.